Nisin It is a polypeptide substance composed of 34 amino acids produced by Streptococcus lactis. It has a strong inhibitory effect on a variety of spoilage bacteria and food pathogens, such as gram-positive bacteria, such as Bacillus, Staphylococcus, Enterococcus, Pediococcus, Lactobacillus, Leuconostoc, Listeria, etc. It is a safe, efficient, non-toxic natural food preservative. In 1969, the FAO/WHO Joint Expert Committee on Food Additives gave international recognition to nisin. So far, nisin has been widely used in more than 70 countries and regions, and has attracted more and more attention. Since it was listed as a food additive supplement in 1992, the application scale of nisin has expanded rapidly. At present, its annual consumption has reached hundreds of tons, and there is still huge market potential. On the other hand, since the beginning of domestic production, the production of nisin in China has also developed very rapidly, and has become the largest producer in the world so far. The market competition also appears and will become increasingly fierce. At present, the quality of nisin products produced by Lanzhou *********** Co., Ltd. has exceeded that of its domestic counterparts and is in a leading position in the world. At the same time, the company is located in the west of China, with climate conditions suitable for fermentation, and has advantages in coal, electricity, human resources, etc. Although the company has only been established for a little more than three years, the nisin produced by the company has been recognized by Yili, Mengniu, Wahaha, Shuanghui and many other well-known domestic dairy and meat products enterprises, and has established a relatively stable cooperative relationship, with sales rising gradually. In addition, the company's nisin products have been exported to the United States, Europe, the Middle East, Southeast Asia, India and Taiwan and other countries and regions. It shows good sales prospects and great development potential. Since its establishment, the company has been working closely with Zhejiang University in production technology and product development, and this project is one of them. According to the biological characteristics of nisin producing strains and the characteristics of nisin production, this project uses the screened gram-negative bacteria and their products to transform the lactic acid produced in the fermentation process into pyruvate through fermentation coupling technology, so as to promote the growth of nisin and increase the production of nisin. At present, the laboratory research work of this project has been completed, and the pilot test has proved that the fermentation potency of nisin can be increased by 30% by changing the traditional fermentation process, without affecting the original separation and extraction process. The technical risk of this project is small and the benefit is improved significantly. The successful development and application of this technology in production will significantly reduce the production cost of nisin and greatly improve the international competitiveness of products; At the same time, the application of nisin in low-end food will become a reality, expand a broader market, and achieve better and faster development of the company.
2、 Lanzhou Fund Application Report Main content:
1. Batch preparation technology of immobilized cells;
2. Hydrodynamics and operation characteristics of the immobilized cell fluidized bed reactor;
3. Kinetics and model of nisin fermentation;
4. Optimization matching design and verification of reaction and transformation coupling system;
5. The high-yield strain of nisin was tested in mass production;
3. Technical level and application scope:
It is the first in the world to use coupling technology to improve the fermentation level. The successful development of this project is a new one for the fermentation industry
Revolution and a new concept breakthrough can be widely used in the preservation of meat products, dairy products, vegetable protein food, canned food, fruit juice drinks and heat treated and hermetically packaged food, as well as in cosmetics, health care products and other fields.
Zhejiang University has a strong technical force and advanced experimental equipment. It has cooperated with our technical personnel in the research of nisin for eight years. The research has successfully extracted and produced nisin with a full physical process, which is unique in the world.
4. On the basis of the original production of our company, the total investment for the expansion of the production line is 10 million yuan, including 5 million yuan for plant construction, 4 million yuan for machinery and equipment, and 1 million yuan for working capital. The above contents are provided by Lanzhou Project Fund Application Report The writing company will provide you with information.
